|Title||Structures and functions of bacterial communities associated to Maldivian seagrass|
|Inizio tesi||Il prima possibile|
|Duarata tesi||6 mesi|
|Descrizione del progetto||
The aim of this thesis project is the characterization, through molecular methods, of potential degradative and depurative abilities towards organic loads of bacterial communities associated to Maldivian seagrass. Leaves of Cymodocea rotundata and Thalassia hemprichii were sampled in Magoodhoo island, from individuals located both in the closeness and far from the area where a groundwater lens is suspected to communicate with seawater, thus promoting wastewater discharge to the sea. The structure of bacterial communities of seagrass and of seawater and sediment from the sea bottom will be characterized through DNA extraction and Illumina sequencing of the taxonomic marker gene coding for 16S rRNA. Quantification of catabolic marker genes involved in the degradation of organic matter will be also performed by quantitative PCR (qPCR).